Emergency crews responded to the area of Main and Water Streets on Route 17.
Officials say Main Street is closed between Foote and Hopewell Roads, but reopened at 11:30 p.m. Tuesday night.
Police say the victim is a female, and died after being transported to the hospital for treatment. There is no word yet if this is a hit-and-run, or simply an accident.
Anyone who may have witnessed the crash is asked to contact the Glastonbury Police Traffic Unit at (860) 633-8301.
This is a developing story. News 8 will bring you any updates as they become available.